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Collecting a TSU Sample

Angus Australia accepts two types of tissue samples for genomic testings; those collected with a Tissue Sampling Unit (TSU) and those collected manually.

TSU sampling requires a setup suitable for ear tagging as the process replicates a process similar to that used for ear tagging animals. The process

requires specialised equipment and collectors in order to perform which are available from your local Allflex supplier.

TSUs are often integrated with ear tags, with the collection tube having a bar code that corresponds to the NLIS and management tag, which helps to prevent human error and sample mix-ups.

TSU Sampling

To collect TSU sample for DNA testing;

1. Arrange TSU collectors and the applicator gun available through your local Allflex supplier and the DNA Test Request form available from Angus Australia.

2. Write on the form or box the registered animal ID next to the corresponding barcode ID

 

 

a. Rotate the black retention ring to the open position

b. Place the TSU into the applicator with the collection tub held within the retention ring. Return the retention ring to the closed position.

c. Squeeze the trigger until the piston engages with the top of the TSU and release.

 

d. Using the

4. With the animal safely restrained, identify the ear to be sampled, ensure it is free from debris and select     a location which avoids veins and any existing identification such as tattoos.

 

 

5. Rapidly depress the trigger of the applicator when positioned correctly on the ear of the animal. Release     the trigger once the trigger has been pulled to the

6. Rotate the black retention ring  to remove the TSU

7. Examine the TSU to ensure the following

a. The cap of the tissue is sealed tightly with no hair obscuring the seal

b. Liquid is present within the TSU with the float ball present

c. The sample can be seen in the liquid

8.  Store in a zip lock bag or TSU holder in a cool, dry place before sending to Angus Australia with the DNA     test request form.

 

TSUs are designed for single use and it is important that the correct procedure is followed to ensure a suitable sample is taken. Importantly the TSU should is checked for a suitable sample and seal prior to being submitted.

 

Dried out / Lost liquid

No sample or seals

The following are real examples of some of the samples which have failed the quality control or DNA extraction process due to issues with the TSU sample.

 

Hair obstructing seal and no sample

Seal stuck in tub cap and insufficient sample